human lambda elisa quantitation  (Fortis Life Sciences)

Journal: Cell chemical biology

Article Title: Stable and potent selenomab-drug conjugates

doi: 10.1016/j.chembiol.2017.02.012

Figure Lengend Snippet: Human multiple myeloma cell line U266-ffluc was i.v. (tail vein) injected into NSG mice. After 4 weeks, the mice were randomized into 3 groups comprising 8–9 mice each and treated with i.v. (tail vein) injections of 3 mg/kg of the ADC, the unconjugated antibody, and vehicle alone. (A) Human lambda light chain concentrations correlating with tumor burden were determined by ELISA at the indicated time points and plotted as mean ± SD values. The p value (t-test) compares the ADC group to the unconjugated antibody group. (B) Kaplan-Meier survival curve. The p value (log-rank test) compares the ADC group to the unconjugated antibody group. See also Figure S3 and Table S3.

Article Snippet: Tumor burden was monitored (i) by weekly in vivo bioluminescence imaging using a Xenogen IVIS Imaging System (Caliper Life Sciences) following i.p. injection of 100 μL of 25 mg/mL D-luciferin (Caliper Life Sciences) and (ii) by weekly measurement of human lambda light chain concentrations using the Human Lambda ELISA Quantitation Set (Bethyl Laboratories).

Techniques: Injection, Enzyme-linked Immunosorbent Assay

Journal: Cell chemical biology

Article Title: Stable and potent selenomab-drug conjugates

doi: 10.1016/j.chembiol.2017.02.012

Figure Lengend Snippet: (A) Human breast cancer cell line KPL-4 was xenografted into the mammary fat pads of female NSG mice, grown to ~150 mm3, randomized into 5 groups comprising 6 mice each, and treated with i.v. (tail vein) injections of the indicated ADCs and controls four times every week at 5 mg/kg. Mean ± SD values are plotted. (B) Four female CD-1 mice were injected i.v. with 6 mg/kg of anti-HER2 scFv-Fc(Ser396Sec)/CN29. The plasma concentrations of total antibody and intact ADC were quantified by ELISA at the indicated time points, using HER2 ECD for capturing and either anti-His mAb (total antibody) or anti-MMAF mAb (intact ADC) for detection. Shown are mean ± SD values for each time point. See also Figure S5, Figure S6, Figure S7, and Table S6.

Article Snippet: Tumor burden was monitored (i) by weekly in vivo bioluminescence imaging using a Xenogen IVIS Imaging System (Caliper Life Sciences) following i.p. injection of 100 μL of 25 mg/mL D-luciferin (Caliper Life Sciences) and (ii) by weekly measurement of human lambda light chain concentrations using the Human Lambda ELISA Quantitation Set (Bethyl Laboratories).

Techniques: Injection, Clinical Proteomics, Enzyme-linked Immunosorbent Assay

Journal: Cell chemical biology

Article Title: Stable and potent selenomab-drug conjugates

doi: 10.1016/j.chembiol.2017.02.012

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Tumor burden was monitored (i) by weekly in vivo bioluminescence imaging using a Xenogen IVIS Imaging System (Caliper Life Sciences) following i.p. injection of 100 μL of 25 mg/mL D-luciferin (Caliper Life Sciences) and (ii) by weekly measurement of human lambda light chain concentrations using the Human Lambda ELISA Quantitation Set (Bethyl Laboratories).

Techniques: Virus, Recombinant, Marker, Conjugation Assay, Proliferation Assay, Enzyme-linked Immunosorbent Assay, Quantitation Assay, Software